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2 edition of Expressed sequence tag analyses of Ustilago maydis teliospore germination. found in the catalog.

Expressed sequence tag analyses of Ustilago maydis teliospore germination.

Nuno Tiago Sacadura

Expressed sequence tag analyses of Ustilago maydis teliospore germination.

by Nuno Tiago Sacadura

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Published .
Written in English


About the Edition

Ustilago maydis grows in its host Zea mays eliciting the formation of tumours that become full of black teliospores. Teliospores are thick-walled, dormant, diploid cells that have evolved for dispersal and survival of the pathogen. Their germination leads to new rounds of infection and is temporally linked to meiosis. I have investigated gene expression during teliospore germination through creation of an EST library. Assembly of 2871 ESTs produced 1293 contiguous sequences. Based upon a blast search similarity cut-off of E ≤ 10-5 38% of all contigs were orphans while 62% showed similarity to sequences in the protein database. Analyses of blast searches were used to functionally classify genes. Northern hybridizations using cDNA clones reveal a relative level of expression consistent with the number of sequences per contig. Identified genes and expression information provide a base for genome annotation of U. maydis and further investigation of teliospore germination and pathogenesis.

The Physical Object
Pagination118 leaves.
Number of Pages118
ID Numbers
Open LibraryOL19747217M
ISBN 100612914097

Introduction: Ustilago maydis is a fungus included in the group of Bacidiomycete that host maize and teocinte plants producing a disease known as common smut or , there are no reports regarding the secretome of this organism grown under in vitro conditions. This information might be useful to characterize the genes involved in several important processes such as those related. Ustilago sensu stricto sub-clade Ustilago davisii sub-clade Ustilago esculenta sub-clade Fig. 1 Phylogram obtained from a phylogenetic analysis by McTaggart et al. (a). Maximum likelihood support values (> 70 %) from RAxML 1 boot-strap replicates and PhyML aRLT values shown above the Size: KB.

Occasionally, abnormal teliospore germination was observed. The most common cytological aberrations were (1) direct teliospore germination from the spore itself or from a single metabasidial cell (Fig. 13), (2) sudden death of a portion or the whole metabasidium, (3) . Microcycle Conidiation in Cercospora zeae-maydis Carrie L. Lapaire and Larry D. Dunkle First author: Department of Botany and Plant Pathology: and second author: Crop Production and Pest Control Research, U.S. Department of Agriculture-Agricultural Research Service, Purdue University, West Lafayette, IN

In the past few years, expressed sequence tag (EST) projects on plant species have generated a vast amount of publicly available sequence data that can be mined for simple sequence repeats (SSRs). However, these EST projects have largely focused on crop or otherwise economically important plants, and so far only few studies have been published. develop microsatellite or simple sequence repeat (SSR) markers for use in population genetics studies, a total of 60, expressed sequence tag (EST) sequences (reads) generated from P. graminis f. sp. tritici were screened for tandemly repeated di- and tri-nucleotide units using a bioinformatics approach and unisequences containing.


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Expressed sequence tag analyses of Ustilago maydis teliospore germination by Nuno Tiago Sacadura Download PDF EPUB FB2

Gene expression and EST analyses of Ustilago maydis germinating teliospores Article (PDF Available) in Fungal Genetics and Biology 40(1) October with 86 Reads How we measure 'reads'. The relevant available expressed sequence tag data is discussed, the databases used in reciprocal best hit blastp analysis are presented and potential U.

maydis meiosis genes are identified. Corn smut is a plant disease caused by the pathogenic fungus Ustilago maydis that causes smut on maize and fungus forms galls on all above-ground parts of corn species. It is edible, and is known in Mexico as the delicacy huitlacoche; which is eaten, usually as a filling, in quesadillas and other tortilla-based foods, and : Ustilaginomycetes.

Ustilago maydis is a model fungal pathogen that induces the formation of tumors in maize. The tumor provides an environment for hyphal differentiation, leading to the formation of thick-walled, diploid teliospores.

Such spores serve as a dispersal agent for smut and rust fungi, and their germination leads to new rounds of infection. The morphological changes that occur during teliospore Cited by: Teliospore germination readily occurs in many species, or it can be induced by treatments such as soaking in water or plant extracts (Fischer and Holton ).

Conjugation can often be seen when compatible cell types are mixed, but the dikaryophase may be unstable in culture and few species have been grown through the complete life history. LARGE-SCALE BIOLOGY ARTICLE The Biotrophic Development of Ustilago maydis Studied by RNA-Seq AnalysisOPEN Daniel Lanver,a,1,2 André N.

Müller,a,1,3 Petra Happel,a Gabriel Schweizer, a Fabian B. Haas,b Marek Franitza,c Clément Pellegrin,a Stefanie Reissmann,a Janine Altmüller,c,d Stefan A. Rensing,b,e and Regine Kahmanna,4 a Max-Planck-Institut für Terrestrische Mikrobiologie, Cited by: Ustilago maydis also serves as an important model for other basidiomycete plant pathogens that are more difficult to work with in the laboratory, such as the rust and bunt fungi.

Genomic sequence of U. maydis will also be valuable for comparative analysis of other fungal genomes, especially with respect to understanding the host range of fungal. The PTEN/PI3K/mTOR signal transduction pathway is involved in the regulation of biological processes such as metabolism, cell growth, cell proliferation, and apoptosis.

This pathway has been extensively studied in mammals, leading to the conclusion that PTEN is a major tumor suppressor gene. PTEN orthologues have been characterized in a variety of organisms, both vertebrates and non Cited by: 1.

Expressed sequence tag analysis of the soybean rust pathogen Phakopsora pachyrhizi Martha Lucia Posada-Buitrago1 and Reid D. Frederick, USDA-Agricultural Research Service, Foreign Disease-Weed Science Research Unit, Ditto Avenue, Fort Detrick, MDUSA Received 5 November ; accepted 10 June Available online 15 November   The maize smut fungus Ustilago maydis switches from yeast to hyphal growth to infect maize (Zea mays) plants.

This switching is promoted by mating of compatible cells and seems to be required for plant penetration. Although many genes distinctively expressed during this dimorphic switch have been identified and shown to be essential for the infection process, none seems to be explicitly Cited by: In this study, Ustilago maydis Ndt80 homolog one, unh1, of the obligate sexual pathogen U.

maydis, is 1 is the sole Ndtlike DNA-binding protein in U. this model basidiomycete, Unh1 plays a role in sexual development, influencing tumor maturation, teliospore development and subsequent meiotic by: 6. Discrete developmental stages during teliospore formation in the corn smut fungus, Ustilago maydis.

Banuett F(1), Herskowitz I. Author information: (1)Department of Biochemistry and Biophysics, School of Medicine, University of California San Francisco,USA.

[email protected] by: Project Information. The Ustilago maydis genome project is part of the Broad Institute's Fungal Genome Initiative and was accomplished through collaborations with the Broad Institute, Bayer CropScience AG and Exelixis, Inc.

The goal of this project was to release 10X genomic coverage for U. maydis strain Bayer CropScience contributed their low-coverage genome sequence of U.

maydis strain   Ustilago maydis is an important fungal pathogen of maize, causing corn smut. It is well adapted to its host and proliferates in living plant tissue without inducing a Cited by: Metabolome and Transcriptome of the Interaction between Ustilago maydis and Fusarium verticillioides In Vitro Wilfried Jonkers,a Alma E.

Rodriguez Estrada,b* Keunsub Lee,b* Andrew Breakspear,a* Georgiana May,b and H. Corby Kistlera,c DepartmentofPlantPathology,UniversityofMinnesota,SaintPaul,Minnesota,USAa;DepartmentofEcology,EvolutionandBehavior,UniversityofMinnesota,SaintPaul.

Abstract. A large-scale comparative genomic analysis of unisequence sets obtained from an Ustilago maydis EST collection was performed against publicly available EST and genomic sequence datasets from 21 species.

We annotated 70% of the collection based on similarity to known sequences and recognized protein by: The hgl1 gene is required for dimorphism and teliospore formation in the fungal pathogen Ustilago maydis. Dürrenberger F(1), Laidlaw RD, Kronstad JW.

Author information: (1)Biotechnology Laboratory, Department of Microbiology and Immunology, and Faculty of Agricultural Sciences, University of British Columbia, Vancouver, BC, V6T 1Z3, by: The genome sequence and gene models of Ustilago maydis were not determined by the Joint Genome Institute (JGI), but were downloaded from NCBI on S In order to allow comparative analyses with other fungal genomes sequenced by the JGI, a.

About the Ustilago maydis genome. Ustilago maydis is the causal agent of the corn smut crop disease. This basidiomycete can infect any part of the plant but usually it reaches the ovaries which causes the kernels to grow into large greyish tumors.

DNA sequence analysis combined with Northern hybridization analysis and primer extension analysis studies has shown that the ornithine-N5-oxygenese gene of Ustilago maydis is complex consisting of one large open reading frame with amino acid sequence similarity to iucD of the Escherichia coli aerobactin operon and three more smaller exonic regions.

This chapter discusses species that include Ustilago hordei in particular, that have provided useful comparative information leading to insights into the genetic basis of bipolar versus tetrapolar mating systems in the smut fungi as a group. It first discusses the importance of smut fungi and the interactions of these pathogens with host plants to provide context for appreciating the role of Cited by: 5.The generation of Expressed Sequence Tags (ESTs) is essential for proper gene prediction in genomes.

Pre-vious Pt EST libraries contributed to gene discovery and stage-specific expression analyses []. Similar EST collections were generated from otherPuccinia species []. However, all these studies were focused on ure.

Puccinia striiformis f. sp. tritici is an obligate biotrophic pathogen that causes leaf stripe rust on wheat. Although it is critical to understand molecular mechanisms of pathogenesis in the wheat stripe rust fungus for developing novel disease management strategies, little is known about its genome and gene functions due to difficulties in molecular studies with this important pathogen.

To Cited by: